Propofol Decreases Myofilament Ca2+Sensitivity via a Protein Kinase C–, Nitric Oxide Synthase–dependent Pathway in Diabetic Cardiomyocytes
PJ Wickley, T Shiga, PA Murray… - The Journal of the …, 2006 - pubs.asahq.org
Background The authors' objective was to assess the role of protein kinase C (PKC) and
nitric oxide synthase (NOS) in mediating the effects of propofol on diabetic cardiomyocyte
contractility, intracellular free Ca2+ concentration ([Ca2+] i), and myofilament Ca2+
sensitivity. Methods Freshly isolated ventricular myocytes were obtained from normal and
diabetic rat hearts.[Ca2+] i and cell shortening were simultaneously measured in electrically
stimulated, ventricular myocytes using fura-2 and video-edge detection, respectively …
nitric oxide synthase (NOS) in mediating the effects of propofol on diabetic cardiomyocyte
contractility, intracellular free Ca2+ concentration ([Ca2+] i), and myofilament Ca2+
sensitivity. Methods Freshly isolated ventricular myocytes were obtained from normal and
diabetic rat hearts.[Ca2+] i and cell shortening were simultaneously measured in electrically
stimulated, ventricular myocytes using fura-2 and video-edge detection, respectively …
